Development of a preliminary diagnostic measure for bovine leukosis in dairy cows using peripheral white blood cell and lymphocyte counts

نویسندگان

  • Masao NISHIIKE
  • Michiyo HAOKA
  • Takashi DOI
  • Tomoko KOHDA
  • Masafumi MUKAMOTO
چکیده

Analysis of the association between antibodies against bovine leukemia virus (BLV), BLV proviral load, and white blood cell (WBC) and lymphocyte counts was performed with 774 dairy cows. The average age, WBC counts and lymphoid cell counts tended to be higher in BLV antibody-positive cows than in antibody-negative cows. There was a similar trend in levels of proviral DNA. We analyzed age, WBC counts and lymphocyte counts by principal component analyses to create a distribution chart of the principle component scores. Using the chart, we categorized cows into four quadrants based on additional information, such as the presence of antibody and the levels of proviral DNA. Antibody-positive cows and cows with high BLV proviral load were found mostly in one quadrant of the chart, indicating that it is possible to predict the risk of infection without any knowledge on antibody status by using information, such as WBC counts as a biomarker. When only antibody-positive cows were included in the analysis, a characteristic distribution of different levels of proviral DNA was seen in the quadrants, suggesting that it is possible to estimate the extent of bovine leukosis infection by using this analysis. For this analysis and categorization of the cows into quadrants, we computed a mathematical formulation using discriminant analysis based on age and WBC and lymphocyte counts. This mathematical formulation for the hematological preliminary diagnosis of the disease is recommended as a screening tool to monitor bovine leukosis.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Influence of the Bovine Acyl-Coa: Diacylglycerol Acyltransferase1 (DGAT1) K232A on Milk Production and Somatic Cell Score Holstein Cows

This experiment was aimed to study the association between the DGAT1 K232A polymorphism and milk production traits and somatic cell score (SCS) in Iranian Holstein dairy cows. The records of 408 animals from five dairy herds were randomly identified and then genomic DNA was extracted from blood using the modified-salting method described by Miller. RFLP-PCR was performed to obtain all the polym...

متن کامل

Prevalence of Bovine Viral Diarrhoea Virus antibodies and antigen among the aborted cows in industrial dairy cattle herds in Mashhad area of Iran

The measurement of antibody responses of animals exposed to BVDV either through a natural exposure or an immunization protocol is still a standard procedure. For BVDV, the test formats have been largely limited to ELISA which is a valuable diagnostic test to measure the level of BVDV specific antibodies as well as antigen in blood samples. In the present study, 120 blood samples were collected ...

متن کامل

Influence of the Bovine Acyl-Coa: Diacylglycerol Acyltransferase1 (DGAT1) K232A on Milk Production and Somatic Cell Score Holstein Cows

This experiment was aimed to study the association between the DGAT1 K232A polymorphism and milk production traits and somatic cell score (SCS) in Iranian Holstein dairy cows. The records of 408 animals from five dairy herds were randomly identified and then genomic DNA was extracted from blood using the modified-salting method described by Miller. RFLP-PCR was performed to obtain all the polym...

متن کامل

The prevalence of bovine viral diarrhea virus in persistently infected cows in industrial dairy herds in suburb of Mashhad- Iran

Bovine Viral Diarrhea Virus (BVDV) is a Pestivirus of the Flaviviviridaefamily that infects cattle, and its widespread distribution can cause substantial economic losses worldwide. The aims of this study were: (1) to estimate the prevalence of animals persistently infected (PI) with BVDV in industrial dairy cattle herds in suburb of Mashhad- Iran; and (2) to compare the occurrence of persistent...

متن کامل

Immunomodulative effects of bovine immunodeficiency-like virus (BIV)-infection and mixed infection of BIV and bovine leukemia virus on sheep.

Experimental bovine immunodeficiency-like virus (BIV)-infection and mixed infection of BIV and bovine leukemia virus (BLV) were performed on sheep. BIV proviral DNA and anti-BIV antibodies were persistently detected in all BIV-inoculated sheep. A slight increase in lymphocyte counts was observed in BIV-infected sheep, but the percentages of CD4+ and CD8+ cells in sheep peripheral blood mononucl...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 78  شماره 

صفحات  -

تاریخ انتشار 2016